Back to Top

Biotech Lab

The Biotechnology Laboratory is set up for all basic and many advanced applications of Molecular and Cellular Biology all performed by undergraduate students.

Molecular Biology techniques are available and equipment for use. Facilities available allow undergraduate and graduate students as well as faculty to use:

Equipment:

  • Refrigerated centrifuge (One clinical refrigerated centrifuge and one medium speed (Fisher 13K Marathon)
  • Beckmann Ultracentrifuge (in basement)
  • Microfuges
  • Micropipettes (including electronic and syringe multidispensing pipettes)
  • Heating blocks
  • UV/VIS Spectrophotometer (Genosys system)
  • Hybridization oven for blots
  • Thermocyclers/Gradient for PCR
  • Incubators (both walk-in (37°C) and bench top (currently 30ºC and 37°C))
  • Walk-in cold room (4°C)
  • Shaking incubators (currently 30°C and 37°C)
  • Shaking platforms
  • Electrophoresis chambers for both nucleic acid and protein gels
  • Transblotter for transfer of proteins or nucleic acids for blots
  • Electroporators
  • Vortexes
  • Sonicator for cell disruption
  • Refrigerators (4°C), frost-free (-20°C) freezer, and (-80°C) freezer
  • Fluorescence microscopes with digital imaging systems
  • Yeast genetics dissecting microscope (with micromanipulator)
  • Digital gel documentation system (Kodak 1D EDAS system)
  • CO2 incubators and BSC hoods for mammalian cell culture
  • Flow cytometer

Techniques that are routinely done in the laboratory:

  • DNA, RNA, and protein isolation
  • Nucleic acid and protein gel electrophoresis
  • DNA, RNA, and protein blots (southerns, northerns, and westerns)
  • Epi-Fluorescence microscopy
  • Enzyme assays
  • Polymerase chain reaction
  • UV/VIS spectrophotometer
  • Flow cytometry
  • Bacterial and fungal culture analysis
  • Mammalian cell culture (in the cell biology teaching laboratory)
  • Fluorescence microscope

Several projects have used these facilities, including:

  • Development of yeast with gene deletions in specific aminopeptidase genes, cell cycle analysis, microarray analysis of gene regulation
  • Analysis of genomic differences in Frankia nodulating plants
  • Analysis of DNA damage due to psoralen exposure
  • DNA typing of rainbow and cutthroat trout
  • Isolation and characterization of acid phosphatase from penicillium
  • Isolation and detection of DNA of pathogens in water supplies

Summer 2022

Two students leaving the OSC

$500 Scholarship Opportunity

Advising for summer courses is open. New and current students are eligible for a $500 summer scholarship if enrolled in 9 or more summer credits. 

Summer Scholarship Interest Form

Schedule Advising

Registration is Open

Student smiling at camera

Registration for both summer and fall courses is open for continuing students. Students can register for classes in self-service Banner or reference the following instructions and resources.

Back to Top