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Biotech Lab

Students from the Biotech Lab have won awards in the several regional Tri Beta Meetings. This includes the sweep of the 1998 conference at the Air Force Academy of the first two places in both Undergraduate and Graduate Divisions.

Students have also won the Eli Lilly award at SACNAS (1998) and the MAC undergraduate award at the American Society for Cell Biology meeting (1999).

The Biotechnology Laboratory is set up for all basic and many advanced application of Molecular and Cellular Biology all performed by undergraduate students.

Molecular Biology techniques available and equipment for use.
Facilities available allow undergraduate and graduates students as well as faculty to use:

Equipment:

  • Refrigerated centrifuge [One clinical refrigerated centrifuge and one medium speed (Fisher 13K Marathon)
  • Beckmann Ultracentrifuge (in basement)
  • Microfuges
  • Micropipettes (including electronic and syringe multidispensing pipettes)
  • Heating blocks
  • UV/VIS Spectrophotometer (Genosys system)
  • Hybridization oven for blots
  • Thermocyclers for PCR
  • Incubators (both walk-in (37oC) and bench top (currently 30oC and 37oC))
  • Walk-in cold room (4oC)
  • Shaking incubators (currently 30oC and 37oC)
  • Shaking platforms
  • Electrophoresis chambers for both nucleic acid and protein gels
  • Transblotter for transfer of proteins or nucleic acids for blots
  • Electroporators
  • Vortexes
  • Sonicator for cell disruption
  • Refrigerators (4o), frost-free (-20oC) freezer, and (-80oC) freezer
  • Epi-Fluorescence microscope with digital imaging system (Zeiss Axioskop with cooled CCD Spot Camera)
  • Yeast genetics dissecting microscope (with micromanipulator)
  • Digital gel documentation system (Kodak 1D EDAS system)
  • CO2 incubator and clean hood for mammalian cell culture
  • Flow cytometer (Epics PROFILE II)

Techniques that are routinely done in the laboratory:

  • DNA, RNA, and protein isolation
  • Nucleic acid and protein gel electrophoresis
  • DNA, RNA, and protein blots (southerns, northerns, and westerns)
  • Epi-Fluorescence microscopy
  • Enzyme assays
  • Polymerase chain reaction
  • UV/VIS spectrophotometer
  • Flow cytometry
  • Bacterial and fungal culture analysis
  • Mammalian cell culture (in the cell biology teaching laboratory)
  • Fluorescence microscope

Several projects have used these facilities, including:

  • Development of yeast with gene deletions in specific aminopeptidase genes, cell cycle analysis, microarray analysis of gene regulation
  • Analysis of genomic differences in Frankia nodulating plants
  • Analysis of DNA damage due to psoralen exposure
  • DNA typing of rainbow and cutthroat trout
  • Isolation and characterization of acid phosphatase from penicillium
  • Isolation and detection of DNA of pathogens in water supplies

Biology Department

Biology Department

Faculty and Staff

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